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fluc plus polya signals  (Addgene inc)


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    Structured Review

    Addgene inc fluc plus polya signals
    (A) Myt3 exons with detectable expression in adult mouse β cells. Only the first 9 of the 25 exons are shown (not in scale). (B) The presence of uORFs in the 5’end of the two primary Myt3 transcripts. (C) Reporter constructs that are used to test stress-induced translation. Note that RLuc and <t>FLuc</t> transcription are all regulated by a TeoO promoter/enhancer, ensuring a constant ratio of their transcription. Also note that in the T2RM construct, a 12 bp sequence (5’-AGCTTTAATGAA-3’) was inserted to disrupt the overlapping uORF. (D) Reporter assay results with or without ER stress response induced by a three-hour heat shock at 42-43 °C. Presented are the ratios between FLuc/RLuc. Each dot represents an independent experiment, each having 2-3 technical duplicates. P-values are from paired t-tests with two-tailed type two errors.
    Fluc Plus Polya Signals, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluc plus polya signals/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    fluc plus polya signals - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Deregulated Myt3 translation predisposes islet β-cells to dysfunction under obesity-induced metabolic stress"

    Article Title: Deregulated Myt3 translation predisposes islet β-cells to dysfunction under obesity-induced metabolic stress

    Journal: bioRxiv

    doi: 10.1101/2025.05.11.653323

    (A) Myt3 exons with detectable expression in adult mouse β cells. Only the first 9 of the 25 exons are shown (not in scale). (B) The presence of uORFs in the 5’end of the two primary Myt3 transcripts. (C) Reporter constructs that are used to test stress-induced translation. Note that RLuc and FLuc transcription are all regulated by a TeoO promoter/enhancer, ensuring a constant ratio of their transcription. Also note that in the T2RM construct, a 12 bp sequence (5’-AGCTTTAATGAA-3’) was inserted to disrupt the overlapping uORF. (D) Reporter assay results with or without ER stress response induced by a three-hour heat shock at 42-43 °C. Presented are the ratios between FLuc/RLuc. Each dot represents an independent experiment, each having 2-3 technical duplicates. P-values are from paired t-tests with two-tailed type two errors.
    Figure Legend Snippet: (A) Myt3 exons with detectable expression in adult mouse β cells. Only the first 9 of the 25 exons are shown (not in scale). (B) The presence of uORFs in the 5’end of the two primary Myt3 transcripts. (C) Reporter constructs that are used to test stress-induced translation. Note that RLuc and FLuc transcription are all regulated by a TeoO promoter/enhancer, ensuring a constant ratio of their transcription. Also note that in the T2RM construct, a 12 bp sequence (5’-AGCTTTAATGAA-3’) was inserted to disrupt the overlapping uORF. (D) Reporter assay results with or without ER stress response induced by a three-hour heat shock at 42-43 °C. Presented are the ratios between FLuc/RLuc. Each dot represents an independent experiment, each having 2-3 technical duplicates. P-values are from paired t-tests with two-tailed type two errors.

    Techniques Used: Expressing, Construct, Sequencing, Reporter Assay, Two Tailed Test



    Similar Products

    fluc plus polya signals  (Addgene inc)
    93
    Addgene inc fluc plus polya signals
    (A) Myt3 exons with detectable expression in adult mouse β cells. Only the first 9 of the 25 exons are shown (not in scale). (B) The presence of uORFs in the 5’end of the two primary Myt3 transcripts. (C) Reporter constructs that are used to test stress-induced translation. Note that RLuc and <t>FLuc</t> transcription are all regulated by a TeoO promoter/enhancer, ensuring a constant ratio of their transcription. Also note that in the T2RM construct, a 12 bp sequence (5’-AGCTTTAATGAA-3’) was inserted to disrupt the overlapping uORF. (D) Reporter assay results with or without ER stress response induced by a three-hour heat shock at 42-43 °C. Presented are the ratios between FLuc/RLuc. Each dot represents an independent experiment, each having 2-3 technical duplicates. P-values are from paired t-tests with two-tailed type two errors.
    Fluc Plus Polya Signals, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluc plus polya signals/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    fluc plus polya signals - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Myt3 exons with detectable expression in adult mouse β cells. Only the first 9 of the 25 exons are shown (not in scale). (B) The presence of uORFs in the 5’end of the two primary Myt3 transcripts. (C) Reporter constructs that are used to test stress-induced translation. Note that RLuc and FLuc transcription are all regulated by a TeoO promoter/enhancer, ensuring a constant ratio of their transcription. Also note that in the T2RM construct, a 12 bp sequence (5’-AGCTTTAATGAA-3’) was inserted to disrupt the overlapping uORF. (D) Reporter assay results with or without ER stress response induced by a three-hour heat shock at 42-43 °C. Presented are the ratios between FLuc/RLuc. Each dot represents an independent experiment, each having 2-3 technical duplicates. P-values are from paired t-tests with two-tailed type two errors.

    Journal: bioRxiv

    Article Title: Deregulated Myt3 translation predisposes islet β-cells to dysfunction under obesity-induced metabolic stress

    doi: 10.1101/2025.05.11.653323

    Figure Lengend Snippet: (A) Myt3 exons with detectable expression in adult mouse β cells. Only the first 9 of the 25 exons are shown (not in scale). (B) The presence of uORFs in the 5’end of the two primary Myt3 transcripts. (C) Reporter constructs that are used to test stress-induced translation. Note that RLuc and FLuc transcription are all regulated by a TeoO promoter/enhancer, ensuring a constant ratio of their transcription. Also note that in the T2RM construct, a 12 bp sequence (5’-AGCTTTAATGAA-3’) was inserted to disrupt the overlapping uORF. (D) Reporter assay results with or without ER stress response induced by a three-hour heat shock at 42-43 °C. Presented are the ratios between FLuc/RLuc. Each dot represents an independent experiment, each having 2-3 technical duplicates. P-values are from paired t-tests with two-tailed type two errors.

    Article Snippet: The cDNAs of RLuc and FLuc plus polyA signals from a vector from Addgene (#226464) were PCR amplified and cloned into a vector containing a bi-directional TetO enhancer/promoter (Addgene, #96963), producing general vector pYW1134.

    Techniques: Expressing, Construct, Sequencing, Reporter Assay, Two Tailed Test